LOCUS       40924_21549        9379 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGFght-Luc DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Development of a transformation system for the edible mushroom 
            Grifola frondosa: demonstrating heterologous gene expression and 
            RNAi-mediated gene silencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9379)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. 
            Ltd., Research and Development; 89 Yokawa, Minamiuonuma, Niigata 
            949-6695, Japan URL :http://www.maitake.co.jp
REFERENCE   3  (bases 1 to 9379)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9379)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. Ltd., 
            Research and Development; 89 Yokawa, Minamiuonuma, Niigata 949-6695,
            Japan URL :http://www.maitake.co.jp"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1500
                     /gene="Gf.GAPDH"
                     /regulatory_class="promoter"
     gene            1..1500
                     /gene="Gf.GAPDH"
                     /label=Gf.GAPDH
     CDS             1501..2523
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      2527..3526
                     /gene="Gf.GAPDH"
                     /regulatory_class="terminator"
     gene            2527..3526
                     /gene="Gf.GAPDH"
                     /label=Gf.GAPDH
     misc_feature    3527..3542
                     /label=multiple cloning site (BamHI-XbaI-SalI) from pUC19
                     /note="multiple cloning site (BamHI-XbaI-SalI) from pUC19"
     regulatory      3543..4269
                     /gene="Gf.TEF3"
                     /regulatory_class="promoter"
     gene            3543..4269
                     /gene="Gf.TEF3"
                     /label=Gf.TEF3
     CDS             4270..5919
                     /label=luciferase
                     /note="firefly luciferase"
     regulatory      5923..6719
                     /gene="Gf.TEF3"
                     /regulatory_class="terminator"
     gene            5923..6719
                     /gene="Gf.TEF3"
                     /label=Gf.TEF3
     primer_bind     complement(6737..6753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6761..6777)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6785..6815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6830..6851)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7139..7727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7901..8758)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8759..8863)
                     /label=AmpR promoter
     primer_bind     9337..9353
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tctgcgcgct tctctcacag ttcacgaagc cgcagataga atgtgtgact ttcatctaca
       61 acgttacact agccgaacag tattccgaac agtcttccga gcagactcag tcctccgagt
      121 ggcaatgtat tctgggcatc ctcagatccc atgcattctc ttcactcaag cggattacct
      181 ggaaaaccgg ccataatgag aagttcgcga atacagtcag gagtgagctg gcctacttgg
      241 atgcgcacgg gatattgcat gtaatccacg gatattgaca gctcgtaatg ggcgttggtg
      301 caggacttcc cgcaggtccg aatgtgtaca tagtgagtga ttcggatgct cctgtggcac
      361 ttctcgggct cacagccgtc ctcagttttg cgaacgccgc ttgttttatt cgccttctca
      421 ttgccttttc tgctgtttgc gttcattacc acacgacgat tttttctgca agctatacaa
      481 ctcatctttc ttcgattaca tgcaagaatc ctgaggtgct tttgcagaaa tctgaccttc
      541 gttcttcgta catacttgtt agccctcgaa ccatcatttt cattctgcat gcacaaaatg
      601 ttgtatttgt aaagtggagg aggctagatt tctctcgaca tatgtatgta gctaagtaaa
      661 actactaccg aagaaactcc cagtcttcta gatcgcctac atcgcgccga agggcgaaat
      721 gtacgactag agctcaaata catcctgtac gtaacctccg ggaagtggca tcttctgaat
      781 tctgaggccg attcggctga aggcgtattg gctcgagaca gggctatgcg tcgggacgtc
      841 gggagcgttc gaagactcca gactgctgat caaggcgctg cgttcgcatt acacacattg
      901 ttcgaacatg tacagagagt ttgacagaca aaacagtaac acttgtttcg agagagacgc
      961 tcgtagctaa acccgatgtc cgaagaggac cctccccacg cgagtccgca agatgaatgc
     1021 gacagttggt tgccacgagc acagagagcg gcaaataccc tcgagtcatc gtacggagct
     1081 tgtcaatcca gcaaatgtac acgagcatgg caggatccaa tcgtaattag gtggctgaga
     1141 tctgaccacc gagaatgtgc gccctagggg atgagtaaac gcacgtttgc gcgtgaatca
     1201 gcgatgatgc tgtacggtgg tgcttagaga tacgaaaagt tgcaagtgaa cgtaaatgga
     1261 ggaaagggac tggttgggaa tattcatgac aagctggcta gaacaagtcg gaaatctagt
     1321 ctgaggcaaa gccaccagcg gagagccgtt cgcggccttg cggtgacagt cgggcaacgg
     1381 ccggaagctg cccggtgtaa tcatccatct tagataacga tcaccacccc accctataag
     1441 acccctctcc atctctgctc ttctccccat ccttcgtctc caaaaccatt atcctcagca
     1501 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     1561 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     1621 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     1681 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     1741 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     1801 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     1861 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     1921 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     1981 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     2041 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     2101 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     2161 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     2221 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     2281 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     2341 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     2401 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     2461 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     2521 gaatagggtg tggagtggat aagagaaaga agtttggtgc tgctgtatga ggactttgcg
     2581 ctacttgtag cttgctgata tgcagtcttg cgtgtttgta caaatgtcaa tagaagcatt
     2641 cggatacctc ttggtggtgt ggttgcgttc gtggtgtttg ccgtcgttgg tgggtgttga
     2701 gagcatccat gtcgcgattg gtcgcgatca tcttgtggca atcttactga atggtgtgag
     2761 catgagcagt tgtcggtgtc gctactatcc tcgcacactt ccatctcgtg gcgttcatct
     2821 tgtggcaaac agtccacgct ggagacaagc gggccagtct cctctacacc tgcgacgccc
     2881 tcagtccaac ttgcttagtt ctgacttcgt tctaccgcac aatggctaca tccgtatgcg
     2941 agctattctc gccatttcca tgtttttgtg caatttctcg aattcgcatg cataattagt
     3001 ccacttgcct ctgtcgaatg catcgcctcc gtccatccca atgacgagac agtgctttca
     3061 caatggtaag ctcattcctt tgcaatgggg cttcgtggcc cttgtactca gaccagtcgt
     3121 cactgaggct aatcaccgtg gcataggaat ctctccacat ttcgctgttt tatagacaga
     3181 tgctgtaaca aatagatttg aggaacctcc gaatgacgat ataattcttc acgccaaggt
     3241 ctcttttttt ttttctccct cgctgtcttt gagcctgatg agaatctcgg cgtttacagt
     3301 atggctcaac ctaccagggc catgttcaca cccttcctgc gaacaacctt tccagtttct
     3361 acgctcattt ccggcagggt tataggagac tgttttgcaa atttcgtgat ttctagtagg
     3421 cgatgtcttc atcttcttgg atcaatgact gaacctgtgt ccgaaccaac aaaggaagtt
     3481 cttcggtcgt gtctcgaaga aacgacaaga ctggttcatc taattggatc ctctagagtc
     3541 gacactgggt ggaagctaat agcaagcatt atgaagctgg tggttatgtg cgtgcccttt
     3601 catttacgtc gatcgatggc acgtcgcaaa catgctcacc ggatcccttc tgcagcaatt
     3661 cacaccgctt agtttcgctc agctgaatga gaagcagtga gatggtaagg ccatagctcg
     3721 gacatgaatc ggatgtcatg tagttgcgat gcatgtatac tcgtagttcc gatgtcggat
     3781 acggcgttag caagaagtgc tgtcatttga acgctgcatg atggcccgtg gggttagctt
     3841 ccccgcattc acatcggatg actgagagac ctggaggcgc ccgaggctga gctggatgtg
     3901 accagcgtga caggacacgt gaatacagtg cattttttcg aagtccgcct gcttacacca
     3961 gcgttatata aaccgagact caacaatccg taaccttatc cccactcttt cctttcctct
     4021 tttcttctat tgtcctatag aaggtggctt ttcttcgtct ctgtcatccc tttcaacagc
     4081 ccgctaactt tttgcgcagg ctccggccaa cctagaaata gagtgctcta tcatccttga
     4141 cgaccttttc gcgtcttatc gcattttaca agtaccccga tcatatcatc gttaaaggtg
     4201 attaccacga cacctaatcc tctcactttt gcaggtctga caatgagatc ggtcgcccag
     4261 atattcgaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg
     4321 gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
     4381 ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc
     4441 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
     4501 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
     4561 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
     4621 atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg
     4681 aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat
     4741 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
     4801 gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac
     4861 tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg
     4921 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
     4981 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
     5041 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt
     5101 caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa
     5161 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct
     5221 cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg
     5281 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
     5341 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
     5401 accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt
     5461 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
     5521 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc
     5581 ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc
     5641 ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc
     5701 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
     5761 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
     5821 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
     5881 atcctcataa aggccaagaa gggcggaaag atcgccgtgt aaacgttgtc cgtcgccgtt
     5941 gttgtttatc ctgtctctat cgctgtgttg ttctatccgg acgtctctct cggctatata
     6001 tctacgtctt gtactctgta ccctagtatt acctctactt cacgcattta tgacatttcg
     6061 cttctttctg ttcgaattgg aggatgatac ggagctagac attgagcgtg aagcagtgtc
     6121 ctcgatgcaa ctgcggagcg attgtcggtg ccacaggcgc gttctgacgg ctgcgcgctc
     6181 accgcaatta ttatttgaat gacggctcct tgaggcttcc aatctctttg cttccaactt
     6241 ccacacttct ggtttcacga cctgcgatgc ccaatgttca tgaataccca gtgcattcgt
     6301 tcgagctgtt cgctcgctcc gcgacgaaca cactcacacc gaacacgact cagcgaaaca
     6361 cgcttatagt cgctgcttgc tatatcctgg ccattgccat tctatggtgc gttccctagc
     6421 ctctgagctt ctgcgagtgg cgtcgaattt gactcttctc aggcatgtgc cgtatctctc
     6481 tgcgatcagt gagttttgca aagttcattg tactttccaa tggactaaac gagttgtttt
     6541 tttttgcgct cagtatatcc ttttaagtga gtacattcag aaaaacgtaa taggacggtg
     6601 aggtctcatt agcatggtag gctattgact gtaggattcc atgaggttag tgcatgttca
     6661 gctttcgagg tgcttcctgg gcttatgaac cgactgcaga tgagccatgc aatcgcagga
     6721 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6781 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6841 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6901 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6961 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7021 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7081 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7141 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7201 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7261 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7321 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7381 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7441 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7501 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7561 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7621 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7681 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7741 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7801 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7861 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7921 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7981 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8041 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8101 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8161 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8221 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8281 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8341 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8401 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8461 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8521 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8581 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8641 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8701 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8761 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8821 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8881 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     8941 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     9001 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     9061 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     9121 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     9181 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     9241 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     9301 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg
     9361 agctcggtac ccggggatc
//
